Literature summary for 6.2.1.64 extracted from

Souphron, J.; Waddell, M.; Paydar, A.; Tokgoez-Gromley, Z.; Martine F Roussel, O.; Schulman, B.
Structural dissection of a gating mechanism preventing misactivation of ubiquitin by NEDD8s E1 (2008), Biochemistry, 47, 8961-8969 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
APPBP1-UBA3 complex bound to NEDD8 mutant A72Q, hanging drop vapor diffusion method, using 0.1 M Tris, 0.4 M ammonium acetate, 9-10% (w/v) PEG 10K, 5 mM dithiothreitol, pH 7.0-8.0	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine	Homo sapiens	-	AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q13564 and Q8TBC4	regulatory and catalytic subunit	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione affinity chromatography and gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine	-	Homo sapiens	AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine	-	?
ATP + [Ubc12]-L-Cys + [NEDD8 protein]	-	Homo sapiens	ADP + diphosphate + [Ubc12]-S-[NEDD8 protein]-yl-L-cysteine	-	-

Synonyms

Synonyms	Comment	Organism
APPBP1-UBA3 complex	-	Homo sapiens
E1 NEDD8	-	Homo sapiens

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Release 2023.1 (January 2023)